蛋白质分子设计。

主要研究基于生物大数据、人工智能、序列及结构模拟等方法解析与基因高效表达以及其稳定性相关的分子特征，并对目标基因进行智能分子设计提高其应用性能。近年来主持国家自然科学基金2项，参与研究课题8项（“863”项目3项，国家自然科学基金5项）。发表研究论文39篇，其中SCI收录论文30篇，授权中国发明专利8项。

发表文章：

1.Guan, Feifei#; Han, Yanshuo#; Yan, Kai; Zhang, Yan; Zhang, Zhifang; Wu, Ningfeng*; Tian, Jian*(Corresponding author); Highly efficient production of chitooligosaccharides by enzymes mined directly from the marine metagenome., Carbohydrate Polymers, 2020, 234: 115909.

2.Zhang, Pingxian, Wang, Yifan, Chachar, Sadaruddin, Tian, Jian*(Corresponding author) and Gu, Xiaofeng* (2020) eRice: a refined epigenomic platform for japonica and indica rice. Plant biotechnology journal, 18, 1642-1644.

3.Li, Qingbin, Yan, Yaru, Liu, Xiaoqing, Zhang, Ziding*, Tian, Jian*(Corresponding author) and Wu, Ningfeng (2020) Enhancing thermostability of a psychrophilic alpha-amylase by the structural energy optimization in the trajectories of molecular dynamics simulations. Int J Biol Macromol, 142, 624-633.

4.Yu, Xiaoxia, Ding, Zundan, Ji, Yangyang, Zhao, Jintong, Liu, Xiaoqing, Tian, Jian*(Corresponding author), Wu, Ningfeng* and Fan, Yunliu (2020) An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. BMC Microbiol, 20, 18.

5.Han Yanshuo, Guan Feifei, Sun Jilu, Wu, Ningfeng*; Tian, Jian*(Corresponding author): Identification of a chitosanase from the marine metagenome and its molecular improvement based on evolution data. Appl Microbiol Biotechnol, 2020, 104(15):6647-6657

6.Qin, Weitong, Zhao, Jintong, Yu, Xiaoxia, Liu, Xiaoqing, Chu, Xiaoyu, Tian, Jian* (Corresponding author) and Wu, Ningfeng (2019) Improving Cadmium Resistance in Escherichia coli Through Continuous Genome Evolution. Front Microbiol, 10, 278.

7.Ding, Zundan, Guan, Feifei, Yu, Xiaoxia, Li, Qingbin, Wang, Qian, Tian, Jian* (Corresponding author) and Wu, Ningfeng (2019) Identification of the anchoring protein SpoIIIJ for construction of the microbial cell surface display system in Bacillus spp. Int J Biol Macromol, 133, 614-623.

8.Yu, Xiaoxia, Wei, Shuai, Yang, Yunxia, Ding, Zundan, Wang, Qian, Zhao, Jintong, Liu, Xiaoqing, Chu, Xiaoyu, Tian, Jian* (Corresponding author) , Wu, Ningfeng, Fan, Yunliu (2018) Identification of cadmium-binding proteins from rice (Oryza sativa L.). Int J Biol Macromol, 119, 597-603.

9.Yan, Yaru, Liu, Xiaoqing, Li, Qingbin, Chu, Xiaoyu, Tian, Jian* (Corresponding author) and Wu, Ningfeng (2018) Effect of rare codons in C-terminal of green fluorescent protein on protein production in Escherichia coli. Protein expression and purification, 149, 23-30.

10.Qin, Weitong, Liu, Xiaoqing, Yu, Xiaoxia, Chu, Xiaoyu, Tian, Jian* (Corresponding author) and Wu, Ningfeng (2017) Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3). RSC Advances, 7, 51460-51465.

11.Tian, Jian, Yan, Yaru, Yue, Qingxia, Liu, Xiaoqing, Chu, Xiaoyu, Wu, Ningfeng and Fan, Yunliu (2017) Predicting synonymous codon usage and optimizing the heterologous gene for expression in E. coli. Scientific reports, 7, 9926.

12.Yue, Qingxia, Yang, Yunxia, Zhao, Jintong, Zhang, Lijie, Xu, Li, Chu, Xiaoyu, Liu, Xiaoqing, Tian, Jian* (Corresponding author) and Wu, Ningfeng (2017) Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge. Bioresources and Bioprocessing, 4:48.

13.Liu, Xiaoqing#, Tian, Jian#(Equal Contribution), Liu, Lihui, Zhu, Tao, Yu, Xiaoxia, Chu, Xiaoyu, Yao, Bin, Wu, Ningfeng and Fan, Yunliu (2017) Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM. Scientific reports, 7, 6786.

14.Wang, Peili, Qin, Weitong, Xu, Jiangtao, Yan Yaru, Tian, Jian* (Corresponding author), Wu, Ningfeng, Yao, Bin: Enhancing the soluble expression of an amylase in Escherichia coli by the mutations related to its domain interactions. Protein expression and purification 2016, 120:35-41.

15.Yu, Xiaoxia, Xu, Jiangtao, Liu, Xiaoqing, Chu, Xiaoyu, Wang, Ping, Tian, Jian* (Corresponding author), Wu, Ningfeng, Fan, Yunliu: Identification of a highly efficient stationary phase promoter in Bacillus subtilis. Scientific reports 2015, 5:18405.

16.Tian, Jian，Woodard, Jaie，Whitney, Anna，Shakhnovich, Eugene(*)，Thermal Stabilization of Dihydrofolate Reductaseusing Monte Carlo Unfolding Simulations and its Functional Consequences，PLoS Computational Biology, 2015, 2015, 11(4):e1004207.

17.Wang, Ping, Huang, Lu, Jiang, Hu, Tian, Jian(*Corresponding author), et al: Improving the Secretion of a Methyl Parathion Hydrolase in Pichia pastoris by Modifying Its N-Terminal Sequence. PLoS One 2014, 9(5):e96974.

18.Tian, Jian, Xu, Li, Zhang, Shuangyu, Sun, Wen, Chu, Xiaoyu, Wu, Ningfeng*: Draft Genome Sequence of the Organophosphorus-Degrading Bacterium Pseudomonas sp. Strain 1-7, Isolated from Organophosphorus-Polluted Sludge. Genome announcements 2014, 2(5).

19.Tian, Jian, Wang, Ping, Huang, Lu, Chu, Xiaoyu, Wu, Ningfeng*, Fan, Yunliu: Improving the thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 using a computationally aided method. Appl Microbiol Biotechnol 2013, 97:2997–3006.

20.Zhu, Tao#, Tian, Jian#(Equal Contribution), Zhang, Shuangyu, Wu, Ningfeng*, Fan, Yunliu: Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03. PLoS One 2011, 6(2):e17367.

21.Su, Yidan#, Tian, Jian#(Equal Contribution), Wang, Ping, Chu, Xiaoyu, Liu, Guoan, Wu, Ningfeng*, Fan, Yunliu: Improving the thermostability of a methyl parathion hydrolase by adding the ionic bond on protein surface. Appl Biochem Biotechnol 2011, 165(3-4):989-997.

22.Tian, Jian, Wu, Ningfeng*, Chu, Xiaoyu, Fan, Yunliu: Predicting changes in protein thermostability brought about by single- or multi-site mutations. BMC bioinformatics 2010, 11.

23.Tian, Jian, Wang, Ping, Gao, Shan, Chu, Xiaoyu, Wu, Ningfeng*, Fan, Yunliu: Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rationally engineering a glycine to proline mutation. FEBS Journal 2010, 277:4901-4908.

24.Shi, Pengjun#, Tian, Jian#(Equal Contribution), Yuan, Tiezheng, et al: Paenibacillus sp. strain E18 bifunctional xylanase-glucanase with a single catalytic domain. Applied and environmental microbiology 2010, 76(11):3620-3624.

25.Tian, Jian, Wu, Ningfeng*, Guo, Jun, Fan, Yunliu: Prediction of amyloid fibril-forming segments based on a support vector machine. BMC bioinformatics 2009, 10 Suppl 1:S45.

26.Tian, Jian, Guo, Xuexia, Chu, Xiaoyu, Wu, Ningfeng*, Guo, Jun, Yao, Bin: Predicting the protein family of methyl parathion hydrolase. International journal of bioinformatics research and applications. 2008, 4(2):201-210.

27.Tian, Jian, Wu, Ningfeng*, Guo, Xuexia, Guo, Jun, Zhang, Junhua, Fan, Yunliu: Predicting the phenotypic effects of non-synonymous single nucleotide polymorphisms based on support vector machines. BMC bioinformatics 2007, 8:450.

28.Tian, Jian, Wu, Ningfeng*, Li, Jiang, Liu, Yajie, Guo, Jun, Yao, Bin*, Fan, Yunliu: Nickel-resistant determinant from Leptospirillum ferriphilum. Applied and environmental microbiology 2007, 73(7):2364-2368.

授权发明专利:

1.壳聚糖酶突变体及其编码基因和应用（专利号ZL202010024623.8）2020年获国家发明专利授权，第2发明人。

2.大肠杆菌漆酶突变体及其编码基因和应用（专利号201510819558.7），2019年获国家发明专利授权，第1发明人。

3.一种启动子和重组表达载体及其用途和表达外源蛋白的方法(专利号：ZL201510149449.9) 2018年获国家发明专利授权，第1发明人。

4.多策略叠加提高甲基对硫磷水解酶在毕赤酵母中分泌表达量的方法（专利号：ZL201510107100.9）2017年获国家发明专利授权，第1发明人。

5.玉米组织特异性启动子及其应用（专利号：ZL201410429204.7），2017年获国家发明专利授权，第1发明人。

6.细胞色素结合结构域蛋白作为助分泌因子提高外源基因在毕赤酵母中分泌表达量的用途（申请号 201210469485.X），2014年获国家发明专利授权，第1发明人。

7.转植酸酶玉米的用途和制备液化液和糖化液及发酵产品的方法（申请号201210415196.1），2014年获国家发明专利授权，第1发明人。

8.有机磷降解酶突变体及其编码基因和应用（ZL201010244452.6），2011年获国家发明专利授权，第4发明人。