Abstract：

Nowadays, with the rapid development of biotechnology, the CRISPR/Cas technologyin particular has produced many new traits and products. Therefore, rapid and high-resolutiondetection methods for biotechnology products are urgently needed, which is extremely important forsafety regulation. Recently, in addition to being gene editing tools, CRISPR/Cas systems have alsobeen used in detection of various targets. CRISPR/Cas systems can be successfully used to detectnucleic acids, proteins, metal ions and others in combination with a variety of technologies, with greatapplication prospects in the future. However, there are still some challenges need to be addressed.In this review, we will list some detection methods of genetically modified (GM) crops, gene-edited crops and single-nucleotide polymorphisms (SNPs) based on CRISPR/Cas systems, hoping to bringsome inspiration or ideas to readers.

KeyWords:

CRISPR/Cas-based detection; trans-cleavage; genetically modified organisms detection;

gene-edited products detection; single-nucleotide polymorphisms detection; challenges and prospects