Abstract：

The CRISPR-Cas system, an adaptive immune machinery evolved by bacteria and archaea to combat phage and foreign nucleic acid invasion, has been harnessed as a revolutionary genome-editing tool in various organisms. However, current applications primarily focus on exploiting its nucleic acid cleavage capability, neglecting its sophisticated adaptive immune mechanisms, such as prespacer acquisition and immunological memory formation. Inspired by the adage "teach a man to fish"—emphasizing self-sufficiency—we propose a paradigm shift: endow plants with the ability to autonomously evolve antiviral immunity by integrating the CRISPR-Cas system’s full adaptive potential.

Key Words: Keywords: CRISPR-Cas system, plant immunity, self-evolution